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O-Level Biology · Nutrition in Humans

Digestion & Human Nutrition

The alimentary canal, digestive enzymes, absorption in the ileum, assimilation and nutritional deficiency diseases — all covered with model answers and a free quiz.

🫁 Pure Biology🇸🇬 Singapore O-Level📝 5 practice questionsUpdated May 2026

Contents

  1. Essential Nutrients
  2. The Alimentary Canal
  3. Digestive Enzymes
  4. The Stomach
  5. The Small Intestine
  6. Villus Structure & Absorption
  7. The Large Intestine
  8. Nutritional Deficiency Diseases
  9. Practice Quiz

1 · Essential Nutrients

NutrientChemical natureFunctionsSources
CarbohydratesSugar, starch, glycogenEnergy source; structural (cellulose in plants)Rice, bread, pasta, fruits
ProteinsAmino acidsGrowth and repair; enzymes, antibodies, haemoglobinMeat, fish, eggs, legumes
Fats/LipidsGlycerol + fatty acidsEnergy store; insulation; cell membranes; fat-soluble vitaminsOils, butter, nuts, meat
VitaminsOrganic molecules (various)Coenzymes, antioxidants, bone health (see deficiencies)Vegetables, fruits, dairy
MineralsInorganic ionsBone/teeth (Ca²⁺), haemoglobin (Fe²⁺), nerve impulses (Na⁺, K⁺)Dairy, meat, leafy greens
WaterH₂OSolvent; reactant in reactions; transport medium; temperature regulationDrinks, fruits, vegetables
Dietary fibreCellulose (indigestible)Adds bulk to faeces; stimulates peristalsis; prevents constipationWhole grains, vegetables

2 · The Alimentary Canal

The alimentary canal is a muscular tube running from the mouth to the anus. Food passes through it by peristalsis — waves of muscular contraction and relaxation that push food along.

Peristalsis

Rhythmic waves of muscular contraction (circular muscles contract, longitudinal muscles relax, then vice versa) that propel food along the alimentary canal. It occurs throughout the oesophagus, stomach and intestines.

Sequence of Organs

Mouth → Oesophagus → Stomach → Duodenum → Ileum → Colon → Rectum → Anus

Accessory organs (not part of the canal but contribute secretions): salivary glands, liver, pancreas, gall bladder.

3 · Digestive Enzymes

EnzymeProduced bySubstrateProductOptimal pH
Salivary amylaseSalivary glandsStarchMaltose~7 (neutral)
PepsinStomach (gastric glands)ProteinPolypeptides~2 (acidic)
Pancreatic amylasePancreasStarchMaltose~7–8
LipasePancreasLipidsFatty acids + glycerol~7–8
TrypsinPancreasProtein / polypeptidesShorter polypeptides~8 (alkaline)
MaltaseSmall intestine (ileum)MaltoseGlucose + glucose~7
PeptidasesSmall intestine (ileum)PolypeptidesAmino acids~7
✅ Exam Tip

The role of bile is commonly tested. Bile is produced by the liver, stored in the gall bladder, and released into the duodenum. It is NOT an enzyme — it emulsifies fats, breaking large fat globules into smaller droplets to increase the surface area for lipase to act on.

4 · The Stomach

The stomach has three main functions:

⚠️ Common Mistake

Students say the stomach "produces acid to digest food." The acid itself does not digest food — it creates the correct pH for pepsin and kills microbes. Only enzymes digest food chemically.

5 · The Small Intestine

The small intestine consists of the duodenum and the ileum. Most chemical digestion is completed here, and virtually all nutrient absorption occurs in the ileum.

In the duodenum, secretions from the pancreas (pancreatic juice: amylase, lipase, trypsin, sodium bicarbonate) and liver (bile) are added. Sodium bicarbonate neutralises the acidic chyme from the stomach, raising pH to ~8 for optimal pancreatic enzyme activity.

6 · Villus Structure & Absorption

Villus (pl. villi)

A finger-like projection of the inner lining of the ileum that massively increases the surface area for absorption. The surface of each villus is further covered in microvilli (the "brush border"), increasing surface area even more.

Adaptations of the Ileum for Absorption

📝 Model Answer: Explain how the structure of a villus is adapted for efficient absorption of glucose (4 marks)

1. The villus has a large surface area (due to microvilli) which increases the rate of absorption by diffusion.

2. The epithelial layer is only one cell thick, reducing the diffusion distance for glucose.

3. A dense network of blood capillaries in the villus constantly removes absorbed glucose, maintaining a steep concentration gradient.

4. Glucose is absorbed by active transport (as well as diffusion) using carrier proteins, allowing absorption even against a concentration gradient.

7 · The Large Intestine

By the time food reaches the large intestine (colon), most digestion and absorption are complete. The main function of the colon is to absorb water from the remaining indigestible material, compacting it into faeces. The rectum stores faeces until they are expelled through the anus.

The colon also contains large populations of bacteria that produce certain vitamins (e.g. vitamin K) and break down some remaining organic matter.

8 · Nutritional Deficiency Diseases

DeficiencyNutrient lackingSymptoms
AnaemiaIron (Fe²⁺)Fewer/smaller red blood cells; fatigue; pale skin; breathlessness
ScurvyVitamin C (ascorbic acid)Bleeding gums; poor wound healing; bruising; joint pain
Rickets (children) / Osteomalacia (adults)Vitamin D and/or CalciumSoft, weakened bones; bowed legs in children
Night blindnessVitamin AInability to see in dim light; dry eyes
KwashiorkorProteinMuscle wasting; swollen abdomen (oedema); poor growth; hair discolouration
MarasmusOverall energy (protein + calories)Severe weight loss; muscle and fat wasting; stunted growth
✅ Exam Tip

Kwashiorkor vs Marasmus is commonly confused. Kwashiorkor = adequate calories but protein-deficient (hence swollen abdomen from oedema). Marasmus = overall starvation (both protein and energy severely deficient, no swelling — just wasting).

🧠 Quick Practice Quiz

8 questions · O-Level style · Instant feedback

Question 1 of 8
Bile is produced by the liver and released into the duodenum. What is the role of bile in digestion?
Bile is not an enzyme and does not chemically digest fats. It emulsifies fats — breaking large globules into smaller droplets — which greatly increases the surface area available for lipase to carry out chemical digestion.
Question 2 of 8
Fatty acids and glycerol are absorbed differently from glucose and amino acids in the ileum. What structure absorbs fatty acids and glycerol?
Fatty acids and glycerol are reassembled into triglycerides and packaged as chylomicrons, which are too large to enter capillaries directly. They enter the lacteal (lymph vessel) and travel through the lymphatic system before entering the bloodstream.
Question 3 of 8
A child shows symptoms of bowed legs and soft bones. Which nutrient deficiency is most likely responsible?
Bowed legs and soft/weak bones in a child are classic signs of rickets, caused by deficiency of Vitamin D and/or calcium. Vitamin D is needed for calcium absorption from the gut.
Question 4 of 8
Which enzyme breaks down starch in the mouth?
Salivary amylase is secreted by the salivary glands and begins the chemical digestion of starch into maltose in the mouth. It works at a near-neutral pH of about 7.
Question 5 of 8
What is the main function of the large intestine (colon)?
The main role of the colon is to absorb water from the undigested food material passing through, compacting it into semi-solid faeces. Most nutrient absorption has already occurred in the small intestine.
Question 6 of 8
Why is it important that digested food molecules are small enough to be absorbed?
Absorption requires molecules to cross the cell membrane of the villus epithelium. Only small, soluble molecules (monosaccharides, amino acids, fatty acids + glycerol) are small enough to pass through. Starch and proteins are too large and must first be digested.
Question 7 of 8
What is the function of hydrochloric acid (HCl) secreted by the stomach?
HCl has three roles: (1) kills most ingested microbes, (2) stops salivary amylase working (it's optimised for pH 7), and (3) provides the low pH (~2) needed by pepsin to digest proteins. HCl itself does not digest food chemically.
Question 8 of 8
Kwashiorkor is caused by protein deficiency. Which symptom distinguishes it from marasmus?
In kwashiorkor, calories are present but protein is severely deficient. Without enough plasma proteins (albumin), blood osmotic pressure falls, causing fluid to leak into the abdomen (oedema/ascites). Marasmus involves overall starvation (both protein and calorie deficient) — no oedema.
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Transport in Humans